English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Plus monthly

Global: Zendy Tools annual

Global: Zendy Tools monthly

Global: Zendy Free Plan

The infection process of the anthracnose fungus Discula theae-sinensis in tea was investigated in detail by fluorescence microscopy. Leaves of the susceptible tea variety ‘Yabukita’ spray inoculated with the conidial suspension were collected sequentially and examined after fluorescence staining with fluorescein-conjugated wheat germ agglutinin. Conidia adhering to the trichomes germinated and formed very short germ tubes. Penetration hyphae grew from the germ tube tips into the cell walls of the trichomes, and formed infection hyphae by 12 h after inoculation. Hyphae that had invaded the mesophyll were confined to the small round spots that formed surrounding the infected trichomes. However, hyphae that had reached the veins extended through the phloem, causing necrosis of the neighboring mesophylls and eventually the formation of large necrotic lesions. Discipline: Plant disease Additional key words: anthracnose, Camellia sinensis, fluorescein-conjugated wheat germ agglutinin Present address: 1 National Institute for Agro-Environmental Sciences (Tsukuba, Ibaraki 305-8604, Japan) *Corresponding author: e-mail kenyama@affrc.go.jp Received 2 September 2013; accepted 22 January 2014. K. Yamada & R. Sonoda 400 JARQ 48 (4) 2014 stems and potted plants were placed in a dew chamber at 26°C for 48 h, and grown at 26°C with a 12-h light/dark cycle. The upper 2-3 leaves of the new shoots on detached stems were sampled at 8, 10, 12, 16, 20, 24 h and 2, 3, 4, 5, 6, 7, 8, 10 days after inoculation and at least 8 leaves on 4 shoots were examined for each sampling period. The experiment was repeated twice. Lesion development after 10 days was observed on intact shoots on potted and field-grown plants, and leaves were sampled depending on the lesion

A Fluorescence Microscopic Study of the Infection Process of Discula theae-sinensis in Tea