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To isolate and measure intact 4-methylthio-3-butenyl glucosinolate (4MTB-GSL) in Japanese radish, we applied a paired ion chromatography method that uses tetradecylammonium ions (TDA) as counter ions. 4MTB-GSL was isolated as ammonium salts from the root of a Japanese radish and identified on the basis of myrosinase treatment, aglycon detection, negative fast atom bombardment (FAB)-mass spectra, and a UV spectrum. The linearity of the prepared standard solution and recovery of that added to Japanese radish extracts were high.

A Chromatographic Method for Separating and Identifying Intact 4-Methylthio-3-Butenyl Glucosinolate in Japanese Radish (Raphanus sativus L.)