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Thirty strains of brewing yeasts were tested for the expression of chromosome dependent killers and the distribution of homologous sequences. Among sake and shochu making yeasts, the frequency of KHR (killer of heat resistant) or KHS (killer of heat sensitive) strains was lower than that of wine making or beer brewing yeasts, but most of them maintained the homolgous sequences of chromosome dependent killer genes. In killer strains, the restriction sites near the killer gene coincided well, but those in non-killer strains did not coincide so well. It was shown that the chromosome dependent killer genes or homologous sequences were not essensial genes fromthe translational expression of KHS and the distribution of KHR homologous sequences.

Distribution of Chromosome Dependent Killer Genes and Homologous Sequence in Brewing Yeasts