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The cells of salt-tolerant yeasts which were Zygosaccharomyces rouxii and Candida versatilis cultivated in the media with and without sodium chloride, and ones of a salt-sensitive yeast which was Saccharomyces sake cultivated in the same media were observed through an optical light microscope and a scanning electron microscope to find out the effect of sodium chloride on their forms and surface structures, and the following results were obtained. (1) The cells of Zygosacch. rouxii cultivated in the medium with sodium chloride were oval, and ones of Zygosacch. rouxii cultivated in the medium without sodium chloride were spherical. The cells of C. versatilis cultivated in the medium with sodium chloride were oval, and ones of C. versatilis cultivated in the medium without sodium chloride were polymorphic. The cells of Sacch. sake cultivated in both medium were oval. When cultivated in the medium with sodium chloride, the cell size of salt-tolerant yeasts became smaller, while that of a salt-sensitive yeast became larger. No difference was found on the surface structures of cells of both types of yeast cultivated in the media with and without sodium chloride, and they were smooth. (2) The chitin of Zygosacch. rouxii cultivated in the medium with sodium chloride existed only on bud scars of cell walls, while the chitin of Zygosacch. rouxii cultivated in the medium without sodium chloride existed not only on bud scars but also on the entire surface of cell walls.

The Cell Forms of the Salt-tolerant Yeast Cultivated in Media with and without Sodium Chloride