English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Plus monthly

Global: Zendy Tools annual

Global: Zendy Tools monthly

Global: Zendy Free Plan

Institute of Biochemistry, Life Sciences Centre, Vilnius University, 7 Saulėtekio Avenue, 10223 Vilnius, Lithuania The aim of this work was to investigate the application of artificial electron transfer mediators for the pyrroloquinoline quinone-dependent soluble glucose dehydrogenase (PQQ-sGDH) catalyzed lactose oxidation reaction. 1-(N,N-dimethylamine)4-(4-morpholine)benzene (AMB) and N,N’-dimethyl-4,4’-azopyridinium methyl sulfate (MAZP) were used as mediators. The mediators showed a high reactivity with a reduced form of PQQ-sGDH and were used to determine the PQQ-sGDH catalyzed lactose oxidation parameters at pH 7.0 and pH 8.0. The use of MAZP and AMB for the regeneration of the oxidized form of PQQsGDH was investigated by applying the electrochemical oxidation of the mediator. The maximum electroenzymatic lactose bioconversion yield and the biggest average PQQ-sGDH turnover number were reached when the buffer solution pH was 8.0 and AMB was used as an electron acceptor, which was oxidized on a carbon fiber electrode of a large surface area.

Electron transfer mediators for PQQ dependent soluble glucose dehydrogenase catalyzed lactose oxidation reaction