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A home-made silica-based silver ion solid-phase extraction (Ag + -SPE) system for the fractionation and subsequent gas chromatographic analysis of trans fatty acids in human adipose tissue is developed and examined. Analytical characteristics of the home-made Ag + -SPE column were compared with those of the commercial Discovery Ag-Ion SPE column and it was demonstrated that the both columns can be applied for the fractionation of fatty acid methyl esters.

Home-made silver ion solid-phase extraction system for the analysis of trans fatty acids: comparison with commercial Discovery Ag-Ion SPE