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A fully-automated HPLC system for simultaneous determination of three pesticides, simazine, thiram and thiobencarb, in tap water was developed.A test tap water was made up in a solution of 2mM sodium ascorbate and 1mM EDTA in order to prevent the decomposition of thiram and thiobencarb from residual chroline and metal ions. Three ml of the tap water injected by the auto sampler was passed through an enrichment column, where the pesticides were trapped quantitatively. The pesticides were then flushed from the column onto an analytic column, where the separation was achieved, and they were measured by the UV detector. The recoveries of simazine (0.3μg/l), thiram (0.6μg/l) and thiobencarb (1.0μg/l) spiked to 7 tap water samples were 93-100%, 92-98% and 81-88%, respectively. It may be possible that many samples are continuously analyzed within a running time of 30minutes by the proposed system. Therefore, it is suggested that the analytical system is effective for daily assay of simazine, thiram and thiobencarb in tap water as a screening method.

Establishment of a Fully-automated HPLC Analytic System for Simazine, Thiram and Thiobencarb in Tap Water.