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The proposal on preparation method of acute aquatic ecotoxicity tests (fish acute toxicity test, daphnia immobilization test and algal growth inhibition test) near the aqueous solubility limit (saturation concentration) through the exposure period is expected in these tests for the poorly water-soluble single organic chemicals. The serial dilution method of water soluble fraction (WSF) and the solvent addition method were substituted and improved using 14 of model poorly water-soluble organic chemical substances having more than 99% purity. The improved solvent addition method was performed as follows: Five test concentration groups were set and the maximum and minimum concentration groups were approximately three times and one third of the apparent solubility, respectively. It was confirmed that the improved solvent addition method could maintain near the apparent aqueous solubility limit of chemical during exposure, was effective for test design of the ecotoxicity test for the poorly water soluble chemicals.

kankyou kagaku/kankyō kagaku

&lt;b&gt;Examination for Preparation of Test Solution in Acute Aquatic Ecotoxicity Test &lt;/b&gt;&lt;b&gt;with Poorly Water-Soluble Single Organic Chemicals &lt;/b&gt;