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Solid-phase high-performance liquid chromatography with electrospray mass spectrometry (LS/MS) was used in this study to analyze pesticides in river water. Polyacrylic resin was used for solid-phase extraction. Simulation was carried out to determine the optimal operation conditions for separation by liquid chromatography. A column for which high reproducibility of retention time even under 100 % aqueous gradient conditions was selected as the liquid chromatography separation column. Cone voltage at which maximal sensitivity was achieved was used for the mass spectrometry. Results of analysis by LS/MS of water samples collected from rivers in Hokkaido, the northernmost island of Japan, revealed seasonal variations in pesticide concentrations in river water and showed that concentrations of herbicides in the river water were generally higher than concentrations of fungicides and insecticides .

Analysis of Pesticides in River Water by High-performance Liquid Chromatography with Electrospray Mass Spectrometry