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The hybridity and genetic purity of pearl millet hybrid Shraddha was analyzed by PCR-based SSR markers in present research. Amongst the 20 primers screened to identify the specific marker associated with hybrid and parental lines, only six primers viz., PSMP-2084, PSMP-2013, PSMP-2202, PSMP-2089, PSMP-2270 and PSMP-2272 were able to produce both female and male parent specific alleles and helped to assess hybridity and genetic purity of the hybrid. SSR marker PSMP-2084 amplified allele size 232 and 455 bp and PSMP-2272 amplified alleles of size 270 and 370 bp which were specific to female parent (RHRB 1A) whereas, alleles of size 343 and 370 bp amplified by PSMP-2084 and PSMP-2272 respectively, were specific to male parent (RHRBI 138). Also, PSMP-2013 amplifying allele at 134 and 180 bp, PSMP-2089 amplifying allele at 142 and 331 bp and PSMP-2202 amplifying allele at 148 and 162 bp were distinguished the hybrid by expression of parent specific alleles. Another marker, PSMP-2270 amplified alleles at 154, 420 and 512 bp in hybrid and both of its parents. Study leads to conclude that, PSMP-2084, PSMP-2013, PSMP-2202, PSMP-2089, PSMP-2270 and PSMP-2272 markers were hybrid specific and can be utilized for hybridity and genetic purity testing of hybrid Shraddha.

Identification of SSR markers for hybridity and genetic purity testing of pearl millet [Pennisetum glaucum (L.) R. BR.] hybrid