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Traditionally, fungal taxonomy was based on observable phenotypic characters. Recent advances have driven taxonomic conclusions towards DNA-based approaches and these techniques have corresponding pros and cons. Species concepts must therefore rely on incorporated approaches of genotypic, phenotypic and physiological characters and chemotaxonomy. Examination and interpretation of morphological characters however vary from person to person. Standardized procedures are used in the taxonomic study of fungi and general practices of phenotypic approaches are herein outlined. It is not possible to detail all techniques for all fungi and thus, this paper emphasizes on microfungi. Specimen collection is the initial step in any Mycosphere 11(1): 2678–2754 (2020) www.mycosphere.org ISSN 2077 7019

Morphological approaches in studying fungi: collection, examination, isolation, sporulation and preservation