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In vitro interaction of three Argentinean isolates of Purpureocillium lilacinum (one isolated from a public park and two from agricultural soils) towards Nacobbus aberrans eggs was studied. After seven incubation days, the three isolates showed reproductive fungal structures fully developed whereas 80 to 100 % of the eggs were infected. No significant differences among the three isolates were observed in fungal activity with respect to hatching and parasitism under the assayed test conditions (p>0.05). The hatching percentage in control plates increased during the evaluation period of the cultures, with no signs of fungal infection, showing significant differences as compared to the plates infected (p<0.05). Although the three isolates studied could be considered as potential agents for biological control of root-knot nematodes, it is necessary to further study some aspects in a wide range of experimental conditions in the laboratory, greenhouse and field so as to determine their real efficiency.

In vitro antagonistic activity of Argentinean isolates of Purpureocillium lilacinum on Nacobbus aberrans eggs