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Citrus root rot causes very high impact on quality and quantity of mandarin cultivation. Phytophthora spp. is root rot causing pathogens. The Phytophthora spp. were isolated from soil and diseased roots of mandarin plants in Chiang Mai province (northern Thailand) and confirmed pathogenicity using detached leaf technique. Arcopolis cupreus species with antifungal activity is an effective strategy for biological control of fungal pathogens. Arcopolis cupreus BN21 was isolated from peanut pods from agricultural waste materials and the isolate was studied in vitro, against the isolated root rot pathogen Phytophthora spp. On dual culture, clear zone of Arcopilus cupreus BN21 against Phytophthora spp. showed antagonistic activity against pathogens. Moreover, the mycelia of BN21 attached to the pathogen hyphae and caused the cell walls degradation of pathogen. Fractional ethanol extract also showed the growth inhibition of the pathogen as well. Adding A. cupreus BN21 suspension into the soil showed the reduction of the root infection form Phytophthora sp.

Efficacy of Arcopilus cupreus as biological agent to control Phytophthora spp. causing root rot of mandarin citrus