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. The amniotic membrane is used in transplant surgery, ophthalmologyand dermatology. Various methods have been developed topreserve amniotic membrane: hypothermic storage, cryopreservation, lyophilization.Transplantation of fresh amniotic membrane showed low inflammatoryresponse. The efficient antibiotic solutions are carefully chosenfor the hypothermic storage of amniotic membranes. The aim of this studywas to compare the efficacy of two antibiotic solutions for the hypothermicamniotic membrane preservation and the structure of the amnioticmembrane after the preservation process.Methods. Fifteen amniotic membranes were prepared and hypothermicallystored in penicillin solution in BSS (2000 IU/ml) while the remainingfifteen in an antibiotic solution containing: benzylpenicillin (50 μg/ml), gentamicin(100 μg/ml), ciprofloxacin (200 μg/ml) and fluconazole (100 μg/ml).All amniotic membranes were microbiologically tested after preparationand after hypothermic storage for two weeks. Histological analysis of thirtyamniotic membranes was performed after the process of preservation.Results. Fifteen amniotic membranes were sterile after hypothermic preservationin the penicillin solution. Also, fifteen amniotic membranes weresterile after hypothermic preservation in the solution of antibacterial agents(penicillin, gentamicin, ciprofloxacin) and antifungal agent fluconazole. Theamniotic membrane had a normal structure and thickness of 35.33±11.03 μm.Conclusion. Both antibiotic solutions, one that contains only penicillinand one that contains multiple antibacterial agents and fluconazole, providesterility of fresh hypothermically stored amniotic membranes for twoweeks. In the preparation of fresh hypothermically stored amniotic membrane,a solution with multiple antibiotics is preferred. The normal tissuestructure of the amniotic membrane was histologically confirmed after theprocess of preservation.

Efficacy of two different antibiotic solutions in preservation of fresh amniotic membrane