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Two enzyme-linked immunosorbent assays ( ELISA) for sulphones in body fluids were adapted to measure sulphones in blood dried on filter paper. The more sensitive modification , based on competition between dapsone (DDS) and enzyme-dapsone conjugate , detects sulphone in blood extracts up till 6 days following 1 00 mg DDS intake . Application to monitor patient compliance is demonstrated, using finger-prick blood from 3 0 Ethiopian leprosy patients. Results are compared t o those in urine , and to statements as regards the last daily dose of 1 00 mg DDS. Eight negative results were found , and employing serial dilutions of positive controls, this indicated that omissions of more than 5 doses in succession occurred. Practical aspects of the technique are discussed.

ELISA inibition technique for the demonstration of sulphones in body fluids. Comparison of two ELISA methods