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Species of the Annonaceae family are used in traditional medicine, and several antitumor acetogenins have been isolated from the leaves of Annona muricata and Rollinia mucosa. Leaves samples (self-shaded and sun-exposed) of these species were collected during winter and summer seasons. Extraction media were prepared from mixtures of five solvents according to a simplex centroid design. Plant extracts were analysed by injection into a high-performance liquid chromatography with diode array detection (HPLC-DAD) and evaluated after applying by multivariate curve resolution-alternating least-squares (MCR-ALS) and discriminant-unfolded partial least-squares (D-UPLS). The objective was to check whether the chemometric resolution of chromatographic profiles could help in the discrimination of samples according to taxonomic classification, sun exposition and/or harvest season. The results indicate that extraction media containing ethanol as main solvent achieved discrimination of self-shaded versus sun-exposed plants and harvest season of Annona muricata. Also, D-UPLS analysis allowed taxonomic discrimination between Annona muricata and Rollinia mucosa extract samples.Fil: Alfonso, Sabrina. Universidade Estadual de Londrina; Brasil. Universidad Nacional de Rosario. Facultad de Ciencias Bioquimicas y Farmaceuticas. Departamento de Quimica Analitica; ArgentinaFil: Pisano, Pablo Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Química Rosario; ArgentinaFil: Silva, Fabiano B.. Universidade Estadual de Londrina; BrasilFil: Scaminio, Ieda S.. Universidade Estadual de Londrina; BrasilFil: Olivieri, Alejandro Cesar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Química Rosario; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquimicas y Farmaceuticas. Departamento de Quimica Analitica; Argentin

journal of the brazilian chemical society

Discrimination of<i>Annona muricata</i>and<i>Rollinia mucosa</i>Extracts by Using Multivariate Curve Resolution and Partial Least-Squares Regression of Liquid Chromatography-Diode Array Data

Discrimination ofAnnona muricataandRollinia mucosaExtracts by Using Multivariate Curve Resolution and Partial Least-Squares Regression of Liquid Chromatography-Diode Array Data