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The fate of natural toxins in the environment has been in focus for the past years. Also, plant produced compounds that can be introduced to the environment by processes such as leaching, decomposition of plant residues or root exudation. The two toxic glycoalkaloids (α-solanine and α-chaconine) and theirs aglycons (demissidine and solasodine) produced by the potato plant (Solanum Tuberosum L.) have been found in upper soil collected each week in two months in varying levels. A new analytical method that uses liquid chromatography Orbitrap mass spectrometry has been developed for the analysis of two glycoalkaloids and two aglycons in potato upper soils, and method validation was performed including sensitivity, recovery, linearity, accuracy and precision and specificity. The application of this method for routine screening of these compounds has been achieved and this study represents a simple, fast and reliable method for the quantification of these compounds in potato upper soils.

Liquid Chromatography-Orbitrap Mass Spectrometry Method for the Determination of Toxic Glycoalkaloids and their Aglycons in Potato Upper Soil