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High resolution magic angle spinning 1H nuclear magnetic resonance spectroscopy (HR-MAS NMR) is a useful technique for evaluation of intact cells and tissues. However, optimal NMR parameters are crucial in obtaining reliable results. To identify the key steps for the optimization of HR-MAS NMR parameters, we assessed different pulse sequences and NMR parameters using sarcoma 180 (S180) cells. A complete assignment of the metabolites of S180 is given to assist future studies.

1H HR-MAS NMR and S180 Cells: Metabolite Assignment and Evaluation of Pulse Sequence

¹H HR-MAS NMR and S180 Cells: Metabolite Assignment and Evaluation of Pulse Sequence