Open AccessDEVELOPMENT AND VALIDATION OF A RP-HPLC METHOD TO DETERMINE THE XANTHYLETIN CONTENT IN BIODEGRADABLE POLYMERIC NANOPARTICLES
Author(s) -
Cristiane de Melo Cazal,
Moacir Rossi Forim,
Maria Fátima das Graças Fernandes da Silva,
Paulo C. Vieira,
João Batista Fernandes
Publication year - 2014
Publication title -
química nova
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.214
H-Index - 73
eISSN - 1678-7064
pISSN - 0100-4042
DOI - 10.5935/0100-4042.20140204
Subject(s) - content (measure theory) , nanoparticle , chromatography , high performance liquid chromatography , materials science , chemistry , nanotechnology , mathematics , mathematical analysis
Xanthyletin is used as an inhibitor of the symbiotic fungus (Leucoagaricus gongylophorus) of the leaf-cutting ant (Atta sexdens rubropilosa), one of the most significant agricultural plague insects. The incorporation of this compound into nanoparticles is a promising approach to effectively control leaf-cutting ants. This study presents the development and validation of a specific analytical method using high-performance liquid chromatography (HPLC) for quantification of the xanthyletin content in biodegradable polymeric nanoparticles. The analytical methodology developed was specific, linear, accurate, precise, and robust. The absolute recovery of xanthyletin in colloidal suspensions was nearly 100%. The HPLC method proved reliable for the quantification of xanthyletin content in nanoparticle formulations
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