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Polygala cyparissias is a plant widespread in Southern Latin America. Recently, we demonstrated the gastroprotective activity of the extract, as well as for one of the isolated metabolites-1,7-dihydroxy-2,3-methylenedioxyxanthone (MDX). In this study, a HPLC method for the quantification of MDX was validated. The HPLC method was linear (0.5-24 µg mL-1 of MDX) with good accuracy, precision and robustness. The content of MDX in the extracts from whole and different parts of the plant ranged from 0 to 5.4 mg g-1 and the gastroprotective index ranged from 72.1 to 99.1%. Thus, the method might be used for the standardization of the extracts based on the MDX marker

química nova

Validation of an HPLC method for the determination of 1,7-dihydroxy-2,3-methylenedioxyxanthone in extracts obtained from<i>Polygala cyparissias</i>A. St. Hil. &amp; Moq. (Polygalaceae) and evaluation of the influence of its content on the gastroprotective activity.

Validation of an HPLC method for the determination of 1,7-dihydroxy-2,3-methylenedioxyxanthone in extracts obtained fromPolygala cyparissiasA. St. Hil. & Moq. (Polygalaceae) and evaluation of the influence of its content on the gastroprotective activity.