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Seroepidemiology, molecular and entomological studies of bluetongue in sheep was carried out in Gujarat, India. Out of 980 sera sample screened, 361 (36.84 %) were found positive by c-enzyme-linked immunosorbent assay (c-ELISA). Out of the 103 blood samples tested for the presence of bluetongue virus (BTV) by reverse transcription-polymerase chain reaction (RT-PCR), 3 were found positive, producing 274 bp amplicons with NS1 gene specific primers. Collection and identification of Culicoides vectors from various farms suggest that the Culicoides oxystoma is the most prevalent species.       Key words: Bluetongue, sheep, c-Enzyme-linked Immunosorbent Assay (c-ELISA), NS1 gene, Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR), Culicoides.

Seroepidemiology, molecular and entomological studies of bluetongue in sheep in Gujarat