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This paper describes a fast and efficient procedure to separate and identify isoquinoline alkaloids from methalonic extract of two Algerian Fumaria (Fumariacea) species (Fumaria capreolata L. and Fumaria bastardi L.) used in traditional medicine in cases of hepatobiliary disfunction and diarrhoea. Total quinolizidine alkaloid contents were 426 mg/100 g (F. capreolata) and 521 mg/100 g (F. bastardi). The isoquinoline alkaloids, stylopine, protopine, fumaritine, fumaricine, fumarophycine, fumariline and fumarofine were determined by gas chromatography – mass spectrometry (GC-MS) in aerial parts of both Fumaria capreolata and Fumaria bastardi. In the first species, an ester of phtalic acid was identified, and in the second species a peak seems to be a benzophenanthridine, probably a dehydro derivative and three other peaks which were identified as phtalidisoquinoline, one of them seems to be dihydrofumariline. The chemotaxonomic significance of the results is discussed.

Determination of isoquinoline alkaloids contents in two Algerian species of Fumaria (Fumaria capreolata and Fumaria bastardi)