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The biological nitrogen fixation (FBN) associated with maize culture becomes extremely important, even if only part of the need for N by culture may be supplied by this process. The aim of this study was to verify the behavior of three maize hybrids to inoculation of Herbaspirillum seropedicae under different nitrogen levels. The test was conducted under controlled conditions of greenhouse and the experimental design was completely randomized design adopted in factorial scheme 3 (hybrid corn) × 2 (plants inoculated and non-inoculated) × 2 (levels nitrogen), with four replicates. Plant height, stem diameter, chlorophyll content, length root, volume of root, content N in shoot and root were determined for 35 days after emergence, as well as the efficiency of use of N. The hybrid BRS 3035 stands out for most of the variables analyzed, producing most of the shoot dry mass, plants with greater height, diameter of stem, root volume and showing the highest rates of N use efficiency. The H. seropedicae was inoculated with promoted increase in the volume of root, root length, dry weight of shoot, chlorophyll content, content of N in aboveground and N use efficiency. Inoculation strain Z-94 of H. seropedicae plus 80 kg ha -1 de N increased the concentration of N in the aerial part of plants of corn by as much as 25% in the evaluated genotypes.

Inoculation of Herbaspirillum seropedicae in three corn genotypes under different nitrogen levels