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Berberine, palmatine, and Phellodendron bark dye was prepared in methanol for HPLC-DAD-MS analysis of liquid dye. Silk was dyed using berberine, palmatine, and Phellodendron bark dye prepared in water. The dye was extracted from the dyed silk using the HCl/methanol/water (2:1:1 v/v/v) solvent system with a slight modification. The liquid dyes and the dye extracted from the silk samples dyed with the three dye sources were examined using the HPLC-DAD-MS analysis to simultaneously detect berberine and palmatine from the plant dye and the dyeings. Colorimetric measurement was carried out using a spectrophotometer to examine the color and the intensities of berberine, palmatine, and Phellodendron bark dyed silk samples. From the liquid dyes, berberine eluted at 5.21 min with the molecular cation m/z

Analysis of Dye Extracted from Phellodendron Bark Using Liquid Chromatography