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Acinetobacter baumannii is non-fermentative gram-negative bacilli which plays an important pathogen especially in intensive care units causing infections and epidemics. Carbapenem resistance often is consisted of OXA-type carbapenemase. In this study, we aimed to determine carbapenem resistance and clonal relationships of A. baumannii isolated from patient and enviromental samples by phenotypic and genotypic methods in 10-bed intensive care unit. Multiplex-PCR method was used to determine the genes of OXA type beta-lactamases (blaOXA) and clonal relations between strains were investigated by pulsed-field gel electrophoresis (PFGE) method. All of the isolates were found to be carbapenem resistant and had the blaOXA-51-like and blaOXA-23-like gene. Also, all of isolates were seen to be 100 % related by PFGE method. As a result, isolates of patients with ventilator-associated pneumonia and isolates survived on ventilator of Intensive Care Unit were found to be 100% clonal associated with PFGE and had same MIC values for imipenem and meropenem. blaOXA-23 and blaOXA-51 genes has been determined all of the isolates. It can be accepted a short-term and small outbreak.

The outbreak of Acinetobacter baumannii producing OXA-23 and OXA-51 type carbapenemases in a state hospital