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Induced mutants (mutational phases) were isolated from representative strains of every serotype of Streptococcus mutans using mechlorethamine HCI. The phases were divided into phase I, phase II, and phase III according to their properties. Phase I induced a firm and adhesive plaque, and severe caries in experimental animals. Phase II showed unstable properties. Phase III did not show adhesive plaque formation, and lost its cariogenecity in experimental animals. In the presence of phases I and III, plaque formation and proliferation in phase I were strongly suppressed in vitro.

A study of mutational phase of Streptococcus mutans: Isolation and properties of mutational phases of Str. mutans.