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In vitro adsorption of dextranase by dental plaque and buccal mucosa was determined, and clinical oral retention of dextranase was also confirmed.Additions of 1320, 6600 and 13200 units of dextranase to dental plaque samples resulted in adsorptions of 2.9, 10.8 and 15.1 enzyme units per 1 milligram dry weight of dental plaque, respectively. Moreover, buccal mucosa adsorbed about 6 units of dextranase per milligram dry weight of buccal mucosa when treated with 16800 units enzyme. The adsorption by buccal mucosa was considered to be weaker than that by dental plaque.After rinsing the mouth for 30 sec. with 13200 units of dextranase, the enzyme activity in the whole saliva was reduced rapidly and disappeared completely after 90min. In spite of this, however, a significant amount of the enzyme activity was found in, the dental plaque of each subject.

IN VITRO ADSORPTION OF DEXTRANASE BY DENTAL PLAQUE AND BUCCAL MUCOSA, AND ITS ORAL RETENTION AFTER RINSING THE MOUTH