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Background:  Plasmid transfer among bacteria provides a means for dissemination of resistance. Plasmid Analysis has made it possible to track plasmids that induce resistance in bacterial population.    Objectives:  To screen the presence of herb-resistance plasmid in  Escherichia coli  strains and determine the transferability of this resistance plasmid directly from  E. coli  to the Gram-positive,  Staphylococcus aureus .    Materials and Methods:  The donor strain  E. coli  CP9 and recipient strain  S. aureus  RN450RF were isolated from UTI patients.  E. coli  CP9 was highly resistant to herbal concoction. Isolates of  S. aureus  RN450RF were fully susceptible. Total plasmid DNA was prepared and transferred into  E. coli  DH5α. Transconjugants were selected on agar plates containing serial dilutions of herbal concoction. Resistance plasmid was transferred to susceptible  S. aureus  RN450RFin triple replicas. The mating experiments were repeated twice.    Results:  The identified 45 kb herb-resistance plasmid could be transferred from  E. coli  CP9 isolates to  E. coli  DH5α. As a consequence  E. coli  DH5α transconjugant MIC increased from 0.0125 g/mL to 0.25 g/mL. The plasmid was easily transferred from  E. coli  CP9 strain to  S. aureus  RN450RF with a mean transfer rate of 1×10 -2  transconjugants/recipient. The  E. coli  donor and the  S. aureus  RN450RF transconjugant contained a plasmid of the same size, which was absent in the recipient before mating. Susceptibility testing showed that the  S. aureus  RN450RF transconjugant was resistant to herbal concoction.    Conclusions:   E. coli  herb-resistance plasmid can replicate and be expressed in  S. aureus .

Transfer of Herb-Resistance Plasmid From Escherichia coli to Staphylococcus aureus Residing in the Human Urinary Tract