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Salmonella  species are among the major pathogens that cause foodborne illness outbreaks. In this study, we aimed to develop a loop-mediated isothermal amplification (LAMP) assay for the rapid and sensitive detection of  Salmonella  species. We designed LAMP primers targeting the  hilA  gene as a universal marker of  Salmonella  species. A total of seven  Salmonella  species strains and 11 non- Salmonella  pathogen strains from eight different genera were used in this study. All  Salmonella  strains showed positive amplification signals with the  Salmonella  LAMP assay; however, there was no non-specific amplification signal for the non- Salmonella  strains. The detection limit was 100 femtograms (20 copies per reaction), which was ~1,000 times more sensitive than the detection limits of the conventional polymerase chain reaction (PCR) assay (100 pg). The reaction time for a positive amplification signal was less than 20 minutes, which was less than one-third the time taken while using conventional PCR. In conclusion, our  Salmonella  LAMP assay accurately detected  Salmonella  species with a higher degree of sensitivity and greater rapidity than the conventional PCR assay, and it may be suitable for point-of-care testing in the field.

Rapid and sensitive detection of Salmonella species targeting the hilA gene using a loop-mediated isothermal amplification assay