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A ready-to-use and self-diffusible dry medium sheet culture system for coliform bacteria, the Compact Dry CF (CDCF) for food, was evaluated . The CDCF was shown to enumerate 26 different strains of coliform bacteria, and to discriminate coliform bacteria from noncoliform bacteria. Of the 26 coliform bacteria that grew on the CDCF , the 26 strains (100%) were blue-green colonies, whereas 8 Gram-negative bacteria other than coliform bacteria formed non-color colonies. Gram-positive bacteria and yeasts did not grow on the CDCF . The CDCF for coliform bacteria from 80 food samples was compared with the pourplate method using violet red bile agar (VRBA), desoxycholate agar (Deso), X-GAL agar (X-GAL), and the 3 M PetrifilmTM CC (PCC) plate method. The correlation coefficients between the CDCF and VRBA, the CDCF and Deso , the CDCF and X-GAL, and the CDCF and the PCC were 0.97, 0.97, 0.97 and 0.99, respectively . The CDCF is useful for the enumeration of coliform bacteria in food and may be a possible suitable alternative to the conventional pour-plate or PetrifilmTM plate methods .

Evaluation of Compact Dry CF Method for Determining Numbers of Coliforms in Food Samples