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Fraser broth and HCLA (Hemolytic ceftazidime lithium chloride agar) were evaluated for the recovery of Listeria spp. and L. monocytogens. Fraser broth required 101-105 CFU/ ml Listeria spp.for the development of a black color at 35°C for 24 hr. The efficacy of Fraser broth 24 hr incubation transfer from EB and EB 48 hr incubation was not different regardless of injury by testing experimentally contaminated, milk and cheese. Out of 301 naturally contaminated meats and meat environmental swabs tested, Fraser broth gave a false-positive rate of 16.0% and a false-negative rate of 1.6% for 24 hr incubation. However false-negative samples were blackend by 48 hr. 100% (50/50) of L. monocytogenes colonies

Determination of Listeria in Food Samples Using Fraser Broth and HCLA Medium.