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Oxygen consumption rate (OCR) of mouse embryoid body (EB) was measured repeatedly in glucose-depleted medium or PBS solution. Non-invasive nature of the scanning electrochemical microscopy allowed sequential OCR measurement of the identical EB sample for more than 24h. We found that the OCR value for the undifferentiated EB (cultured for 2 days) reached almost zero after 12h exposure in PBS. On the contrary, the OCR value for the differentiated EB (cultured for 8 days) indicated 40 to 28% normal to the OCR at 0h-incubation after 24h exposure in PBS-based solution. It is suggested that differentiated cells indicated a stronger adaptation to glucose deprivation comparing to the undifferentiated cells. © The Electrochemical Society of Japan, All rights reserved.

Sequential Monitoring of Oxygen Consumption Rate of Mouse Embryoid Bodies in Glucose-Depleted Solution