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We propose an electrochemical, low-concentration adenosine triphosphate (ATP) assay combined with enzymatic ATP amplification. The electrochemical ATP assay consists of four parts: (i) myokinase for converting adenosine monophosphate (AMP) + ATP to two adenosine diphosphate (ADP) molecules, (ii) pyruvate kinase for converting two ADP molecules back to two ATP molecules (ATP amplification) and conversion of phosphoenolpyruvate to pyruvate, (iii) pyruvate dehydrogenase for generating H2O2 coupling with pyruvate oxidation, and (iv) electrochemical detection of H2O2. This system was used to detect ATP with a detection limit of 10−9M ATP. © The Electrochemical Society of Japan, All rights reserved.

Sensitive Electrochemical ATP Assay Combined with Enzymatic ATP Amplification Reaction