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An immunoliposome encapsulating a Ru complex with two aminobutyl moieties was prepared to rapidly detect bovine serum albumin (BSA) protein. The detection was carried out within 80 min by electrochemiluminescence (ECL) from the Ru complex adsorbed on Au electrodes after immunoreaction. The adsorption of the complex is a key-step of this procedure in order to increase its sensitivity. Under optimal conditions, the present method can detect BSA in the concentration range from about 1×10−9 g/mL to 1×10−6 g/mL, with almost the same sensitivity as that obtained by conventional ELISA.

Rapid Detection of BSA Protein by Electrochemiluminescence Sensor Combining an Immunoliposome Which Encapsulates a Ru Complex