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Glucose sensing with use of glucose oxidase, p-acetamidophenol, and Nafion-coated Glassy carbon electrode has been attempted aiming quantitative analysis of glucose without interference of electrochemically active species such as ascorbic acid, uric acid, and p-acetamidophenol. Electrochemical measurements of the three interfering species at a Nafion-coated glassy-carbon electrode have revealed that the electrode can oxidize only p-acetamidophenol. It has been also found that p-acetamidophenol works well as an electron mediator for glucose oxidase. Combination of those facts led to fabrication of a glucose sensor that avoids any interference by ascorbic acid and uric acid. When concentration higher than 5.0 mM is chosen for p-acetamidophenol as the electron mediator, further addition of p-acetamidophenol gives no significant changes in the oxidation currents. In addition, even oxygen dissolved in solution does not influence magnitude of sensing currents. Therefore, the glucose sensor prepared in this study is not disturbed by all electrochemically active substances which usually give the most serious interferences for oxidative glucose sensing.

Glucose Sensing Using p-Acetamidophenol as an Electron Mediator between Glucose Oxidase and Nafion-Coated Glassy Carbon Electrode