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In this work, we described the development an electrochemical sensor based on electroreduction of graphene oxide (rGO) on a glassy carbon electrode (GCE) for simultaneous determination of l-Dopa and benserazide. For the elaboration of the GCE/rGO, the developed methodology was based on the electrochemical technique: multiple pulse amperometry (MPA). The MPA was more stable and efficient for the formation of rGO film, under optimum conditions (pH 6.00; concentration of rGO 2.00 mg mL; time 450 s; potentials −0.60, −0.70, −0.80, −0.90, −0.95, −1.00, −1.10, −1.20, and – 1.30 V). After the film was formed, the cyclic voltammetry was used to detect LD and BZ in real samples and optimized conditions 0.05 mol L PBS (pH 5.50). The linear range for the LD is 25–425 μmol L and the BZ of 5–80 μmol L. The limit of detection calculated was 17.10 (LD) and 2.99 (BZ) μmol L.

intechopen ebooks

An Electrochemical Sensor Based on Electroreduction of Graphene Oxide on a Glassy Carbon Electrode Using Multiple Pulse Amperometry for Simultaneous Determination of L-Dopa and Benserazide