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Many serious problems, such as artificial control and overworking of honey bee colonies, deterioration of bee products due to incorrect treatment and inadequate environments, have been postulated in recent beekeeping which should be resolved for sustainable development of modern highly profitable beekeeping in the future. Thus, a novel beekeeping method, Kikuji Yamaguchi Method of Natural Beekeeping (KYAMENABEE), was established for the natural royal jelly (RJ) preparation and the biological and pharmacological properties were examined for evaluation of the authenticity of royal jelly products. RJ samples prepared by KYAMENABEE and ordinal beekeeping were subjected to the quantitative analyses of 10HDA and MRJP1 multimer, identification of functional substance based on the effective growth and development of queen bees, stability of the functional substance, proliferative activity of human and animal cells. The content of 10HDA and MRJP1 multimer in RJ prepared by KYAMENABEE were significantly higher than that prepared by ordinal beekeeping. The biological and pharmacological activities were also superior for RJ prepared by KYAMENABEE than that by ordinal beekeeping. Thus, it might be important to use a novel beekeeping method, KYAMENABEE, in order to produce high quality RJ for sustainable development of biopharmaceutical beekeeping.

Kikuji Yamaguchi Principles of Natural Beekeeping: A Novel Bio-Method of Natural Beekeeping for High Quality Royal Jelly Production