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This study is an attempt to assess, evaluate and compare the spectral difference in saliva and serum between healthy and anomalies pregnant women because of deficiency of folate by utilizing Fourier Transform Infrared Spectroscopy. Folate is required for the development of healthy embryo and plays vital role in the fetus spinal cord and brain development. The present work is to study the folate deficiency in pregnancy-Anomalies (open neural defect) and contrast the outcome of the result with normal healthy pregnant women. The outcome of the results showed that there is a significant difference or contrast between the folate of healthy pregnant and anomalies (open neural defect) in pregnant women, both in the sample of saliva and serum. From the spectral analysis, the intensity ratio parameters have been computed and introduced. The result of the outcomes shows that for both qualitative and quantitative investigation of biological fluids and to distinguish between the sample sets from healthy and anomalies-diseased groups, FTIR is utilized. The internal standard method is described in characterizing the samples quantitatively.

Deficiency of Folate in Pregnancy on Diverse Subjects Using FTIR Spectroscopy