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Animal production is negatively impacted by global warming, is subject to serious consequences for livestock production systems. In order to understand how PBMCs of Indian Zebu-Jersey crossbreds respond to various levels, durations of thermal assault, heat shock, in this chapter we will discuss techniques involving in vitro thermal stress stimulation (TSS) to stimulate bovine peripheral blood mononuclear cells (PBMCs) under various thermal assault conditions (TACs), including normal to extreme temperatures, varying durations of thermal exposure (DTEs). The consequences of thermal stress on bovine species can be lessened, managed with an understanding of how PBMCs as a cellular system respond to in vitro heat shock, thermal assault. To learn more about how Indian Zebu-Jersey crossbreds respond to in vitro thermal conditions, it may also be possible to explore the relationship between the decrease in PBMCs count during in vitro TSS, the expression of the heat shock protein genes (HSPs) such as HSPs 70, 90 genes. This will be exploited to discover how Indian Zebu-Jersey crossbreds respond in vivo to diverse environmental thermal conditions, will further enable in vivo understanding of the potential for thermotolerance in bovine species for better adaptability, survival,, production performance.

veterinary medicine and science

Techniques of Using Peripheral Blood Mononuclear Cells as the Cellular System to Investigate How of the Bovine Species (Indian Zebu-Jersey Crossbreds) Responds to in vitro Thermal Stress Stimulation (Thermal Assault/Heat Shock)