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During the last decades, a great interest has been shown for miniaturised separation tech‐ niques. The use of microfluidic techniques fulfills the constant needs for increasing sam‐ ple throughput and analysis sensitivity, while reducing costs and sample volume consumption. In this chapter, three microfluidic separation techniques will be addressed: capillary electrophoresis, gas chromatography and liquid chromatography. A special at‐ tention will be paid to miniaturised liquid chromatography, with a deep investigation of its advantages compared with classical liquid chromatography. Sample preparation adapted to low volumes (a few μl) will also be discussed.

Advances in Low Volume Sample Analysis Using Microfluidic Separation Techniques