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Hybridization is a key phenomenon in bonding resin-based composite restorations to dentin, and results from a molecular-level interaction between the resin and the demineralized collagen fibrils network. Nanoleakage occurs when small molecules or ions infiltrate into the hybrid layer. Our work aims to evaluate if the type of solvent and adhesive system influences the morphology of the hybrid layer and the occurrence of nanoleakage within it. Human molar teeth were distributed into six groups corresponding to adhesives with different solvents (ScotchbondTM 1XT, XP BondTM, Prime&Bond® NT, One Coat Bond®, AdheSE® and Xeno® V). Dentin disks (specimens) were cut from those teeth. The corresponding adhesive systems and a microhybrid composite (Synergy® D6) were applied onto them. The specimens were thermocycled, fixed, cut, polished, decalcified, dried, and, for nanoleakage evaluation, immersed into a tracer solution. The morphology and nanoleakage analyses were performed with a highresolution scanning electron microscope (field-emission scanning electron microscope —FESEM), and the results were statistically processed. AdheSE® achieved the overall best performance. The type of adhesive, the number of steps, and the solvent seem to play a significant role in hybrid layer morphology and nanoleakage within it. The hybrid layer water absorption can determine the adhesion longevity.

Etch-and-Rinse and Self-Etch Adhesives Behavior on Dentin