English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Plus monthly

Global: Zendy Tools annual

Global: Zendy Tools monthly

Global: Zendy Free Plan

The ICL1 gene, which encodes the glyoxylate cycle enzyme isocitrate lyase (Icl1), is re‐ quired for the growth of Candida albicans on non-fermentable carbon sources and for this yeast to be virulent. The aim of this study is to test the stability of the Icl1 enzyme in re‐ sponse to glucose. Glucose was found to trigger the degradation of the ICL1 but the CaIcl1 was not destabilized by glucose. When CaIcl1 was expressed in Saccharomycess cer‐ evisiae, it was not degraded in response to glucose, suggesting that CaIcl1 has lost the mo‐ lecular signal that triggers destabilization in response to glucose. However, when ScIcl1 was expressed in C. albicans it was rapidly degraded in response to glucose indicating that C. albicans has retained the molecular apparatus for glucose-accelerated degradation of target proteins. ScIcl1 degradation was slowed in Caubi4/ubi4 in which ubiquitin-medi‐ ated protein turnover is reduced. Furthermore, the addition of putative ubiquitination site to the carboxyl-terminus of CaIcl1 led to the glucose-accelerated degradation of this protein. C. albicans has retained the apparatus for ubiquitin-mediated degradation of tar‐ get protein in response to glucose. However, CaIcl1 has lost the Ubi-site that mediate glu‐ cose accelerated protein degradation, thereby allowing C. albicans to simultaneously assimilate alternative carbon sources and glucose.

Metabolic Adaptation of Isocitrate Lyase in the Yeast Pathogen Candida albicans