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Yeasts are the favorite alternative hosts for the expression of heterologous proteins for research, industrial or medical use [1]. As unicellulars microorganism have the advantages of bacteria as ease of manipulation and growth rate. But comparing with bacterial system, they are capable of many of the post-translational modifications performed by higher eukaryotic cells, such as proteolytic processing, folding, disulfide bond formation and glycosylation [2].

Bioprocess Engineering of Pichia pastoris, an Exciting Host Eukaryotic Cell Expression System