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Confocal laser scanning microscopy (CLSM) is a useful image tool to study the fate of delivery systems and biomolecules applied into the skin. Through the use of fluorescence probes, it is possible to evaluate their behavior, like: i) interaction with the biological system [1]; ii) cellular uptake [2-3]; iii) depth of penetration [4]; iv) penetration routes into the skin [5]; v) quantifi‐ cation of skin penetration of drugs and biomolecules [6]; and vi) effect of topical therapies for several skin diseases by morphological analysis of the tissue [7].

Confocal Laser Scanning Microscopy as a Tool for the Investigation of Skin Drug Delivery Systems and Diagnosis of Skin Disorders