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Pulsed Field Gel Electrophoresis (PFGE) has been widely applied to characterize numerous bacteria. PFGE is a form of RFLP typing in which the bacterial genome is digested with rare cutting enzymes. These restriction enzymes cut genomic DNA infrequently and thus generate a smaller number of DNA fragments (10-20 bands). These fragments of a wide range of sizes, from 20 kb to 10,000 kb (Herschleb et al., 2007), are separated using specialized electrophoresis techniques. Differences in the restriction profiles are used to carry out genetic comparisons among isolates. Computer-based analysis is simplified, enabling rapid and easy comparison on strains. Currently, PFGE is often considered the”gold standard” of molecular typing methods for bacterial foodborne pathogens such as Salmonella, E.coli, Campylobacter, Yersinia, Vibrio and Listeria.

The Use of Pulsed Field Gel Electrophoresis in Listeria monocytogenes Sub-Typing - Harmonization at the European Union Level