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Chagas disease, also known as American trypanosomiasis, has its epidemiology conditioned to the (i) triatominae vectors, (ii) etiologic agent, Trypanosoma cruzi, and (iii) sylvatic and sinantropic reservoirs, the mammals. Social factors associated with economic factors, such as industry development, population growth and rural area colonization, which lead directly to ecological imbalance, provide favorable conditions for the disease establishment (Barretto, 1967; Avila-Pires, 1976).

Molecular and Proteolytic Profiles of Trypanosoma cruzi Sylvatic Isolates from Rio de Janeiro-Brazil