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Proteomics is the large-scale study of an organism's complete complement of proteins, and its relevant technologies have matured over recent years. Along with the development of mass spectrometry (MS), MS-based proteomics has emerged as an invaluable tool for largescale identification and quantification of protein networks (Aebersold & Mann, 2003; Domon & Aebersold, 2006). Proteomic data is important for a wide range of research in basic and medical biology. In recent years, many large-scale projects have been performed and a huge amount of data has accumulated. However, because the data sets from individual projects often vary in quality, the value of proteomics for the wider scientific community is limited (Olsen & Mann, 2011).

One-by-One Sample Preparation Method for Protein Network Analysis