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This work was initiated by the problem of investigating the photophysical properties of complex protein molecules and performing the diagnostics of such molecules in water environment. At the present time, fluorescence spectroscopy (fluorimetry) is widely used to study complex organic compounds (COC) (Lakowicz, 1999). Together with spectrophotometry these methods form the basis for fast and nondestructive diagnostics of COC in the natural environment, i.e. they present the diagnostic methods in vivo and in situ. However, the conventional (linear) fluorescence spectroscopy methods can not provide complete information on fluorescent objects under study because of insufficient selectivity (fluorescence bands of most COC are broad and structureless at room temperature).

Laser Fluorescence Spectroscopy: Application in Determining the Individual Photophysical Parameters of Proteins