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The interactions of light with a leaf are determined by the three-dimensional internal and external leaf structure as well as photosynthetic pigment content within the cells (Gamon and Surfus, 1999). The three-dimensional structure and pigment content of a leaf is determined by a number of factors including plant species and varieties within species (genetics), leaf age, and growth conditions including abiotic and biotic stress factors, hormonal activity, and light history (Gamon and Surfus, 1999; Nath et al., 2003). Stresses such as water loss, suboptimal temperatures, high soil salinity, disease, arthropod feeding damage, nutrient deficiency, chemical pollution, and micronutrient toxicities may alter the internal structure of leaves (Gausman, 1985).

Accurate Spectral Measurements and Color Infrared Imagery of Excised Leaves Exhibiting Gaussian Curvature from Healthy and Stressed Plants