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Since the first invention back in 1930s (Tiselius, 1937), electrophoresis methods have diversified significantly and new techniques along with applications are still being developed till date. This method, through agarose or polyacrylamide gels, is the most commonly used for sorting of biological macromolecules such as deoxyribonucleic acid (DNA), ribonucleic acid (RNA), or protein, from both bacteria and viruses. Though agarose gels have a lower resolving power than polyacrylamide gels, they can separate DNAs from 50 bp to several mega bases in length on gels of various concentrations and configurations. Polyacrylamide gels are most effective for separation of small DNA fragments (5 to 500 bp).

Discriminatory Power of Agarose Gel Electrophoresis in DNA Fragments Analysis